BD, 557995, BD Pharmingen™ Alexa Fluor® 700 Mouse Anti-Human IFN-γ

Alternative Name: IFNG; Interferon-gamma; Interferon-γ; Type II interferon; MAF
Reactivity: Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Isotype: Mouse IgG1, κ
Immunogen: Human IFN-γ Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_396977
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed.
Recommended Assay Procedures: Recommended Assay Procedures The Alexa Fluor® 700-conjugated B27 antibody (Cat. No. 557995) antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate IFN-γ producing cells within mixed cell populations. For optimal immunofluorescent staining for flow cytometric analysis, the anti-cytokine antibody should be titrated (≤ 0.25 µg mAb/million cells). For specific methodology, please visit the protocols section under "Intracellular Flow" or "Cytokines (Intracellular Staining)" on our web site, http://www.bdbiosciences.com/us/s/resources. A useful control for demonstrating specificity of staining is the unlabeled B27 antibody used to pre-block the fixed/permeabilized cells (Cat. No. 554699) prior to staining. A suitable mouse IgG1 isotype control for assessing the level of background staining is Alexa Fluor® 700-conjugated mAb MOPC-21 (Cat. No. 557882).