BD, 558074, BD Pharmingen™ APC Mouse Lineage Antibody Cocktail, with Isotype Control

Reactivity: Mouse (QC Testing)
Application: Flow cytometry (Routinely Tested)
Regulatory Status: RUO
RRID: AB_1645213
Description: Description The APC Mouse Lineage Antibody Cocktail has been designed to react with cells from the major hematopoietic lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, NK cells, erythrocytes, and granulocytes. This pre-diluted Cocktail of five APC-conjugated antibodies is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include clone 145-2C11, which recognizes Mouse CD3e; M1/70, which  recognizes CD11b; RA3-6B2, which recognizes CD45R/B220; TER-119, which recognizes Ly-76, mouse erythroid cells; and RB6-8C5, which recognizes Ly-6G and Ly-6C. APC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative-control immunoglobulin. Additional fluorochrome-labeled reagents may be combined with the APC Mouse Lineage Antibody Cocktail, and the APC Mouse Lineage Isotype Control Cocktail, to further characterize hematopoietic progenitor subpopulations.
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Recommended Assay Procedures: Recommended Assay Procedures 20 µl of the antibody or isotype control cocktail is sufficient to stain each sample of 10^6 leukocytes for flow cytometric analysis. We recommend the use of Mouse BD Fc Block™ purified anti-mouse CD16/Cd32 (Cat. No. 553141/553142) for optimal staining. The APC Mouse Lineage Antibody Cocktail can be used to deplete cells bearing the hematopoietic lineage markers by flow cytometric or immunomagnetic (using BD™ IMag anti-Allophycocyanin (APC) Particles - DM, Cat. No. 557932) sorting. The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.