BD, 558937, BD Pharmingen™ PE Mouse Anti-Human CD119

Alternative Name: IFN-γ Receptor α; IFN-γRα; IFNGR1; IFNGR; IFN-gamma receptor 1/IFN-gamma-R1
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Human IFN-γRα
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_397165
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent staining and flow cytometric analysis: PE Mouse Anti-Human CD119 (Cat. No. 558937) can be used for the immunofluorescent staining (≤ 1 µg antibody/10[6] cells) and flow cytometric analysis of the levels of membrane IFN-γRα expressed by human cell lines or human lymphoid cells. An appropriate immunoglobulin isotype control is PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058). Since GIR-94 is a non-neutralizing antibody, it can be used for the unobstructed immunofluorescent staining and flow cytometric analysis of cells in systems where the IFN-γ ligand is present. Based on our testing results,(data not shown) the presence of exogenous recombinant human IFN-γ at levels ≤ 50 ng/10[6] cells was insufficient to inhibit the binding of GIR-94 (at 0.06 µg mAb/1 million cells).