BD, 559048, BD Pharmingen™ Purified Mouse Anti-Human CD57

Reactivity: Human (QC Testing)
Isotype: Mouse IgM, κ
Immunogen: Membrane extracts of the HSB-2 T-lymphoblastoid cell line
Application: Flow cytometry (Routinely Tested), Functional assay, Immunohistochemistry-paraffin, Western blot (Reported)
Concentration: 1.0 mg/ml
RRID: AB_397184
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Functional Studies: Anti-HNK-1 monoclonal antibody recognizes a subset of NK lymphocytes and T lymphocytes. Unlike the populations of cells recognized by Anti-N-CAM monoclonal antibodies that share the unique ability to mediate direct, non-major histocompatibility complex-restricted cytotoxicity against certain tumor cells, the T lymphocytes recognized by anti-HNK-1 monoclonal antibody do not have NK activity. Anti-HNK-1 monoclonal antibodies can be used with other anti-N-CAM monoclonal antibodies to better understand the role of HNK-1 and N-CAM in leukocyte cytolytic activity against tumor cells. Anti-HNK-1 monoclonal antibody can be used in studies on the role of N-CAM and HNK-1 in the development and regulation of neuroendocrine tissues and muscle regeneration. Immunohistology: Anti-HNK-1 monoclonal antibody can be used to stain paraffin-embedded tissues. Immunoblotting: Anti-HNK-1 monoclonal antibody can be used in Western blot analysis.
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.