BD, 559277, BD Pharmingen™ Purified Hamster IgG2, κ Isotype Control

Alternative Name: Anti-KLH
Isotype: Armenian Hamster IgG2, κ
Immunogen: Keyhole Limpet Hemocyanin
Application: ELISA Standard (Routinely Tested), Flow cytometry, Isotype control (Tested During Development), Immunohistochemistry, Intracellular staining (flow cytometry) (Not Recommended)
Concentration: 0.5 mg/ml
RRID: AB_397213
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures This immunoglobulin is useful as a standard in ELISA as an isotype-matched negative control for immunofluorescent staining of unfixed, live cell suspensions. An isotype control should be used at the same concentration as the antibody of interest. This product is not recommended for use as an isotype control for intracellular or immunohistochemical staining applications.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.