BD Pharmingen™ Purified Mouse Anti-Rat OX40 Ligand (CD252) 559957

Alternative Name: Tnfsf4; OX40 ligand; OX40L; TXGP1; Txgp1l; gp34; Ath-1; Ath1; CD134L
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: ACI-S1 rat T-cell lymphoma cell line
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_397393
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures We recommend, as a second-step, the biotinylated goat anti-mouse Ig polyclonal antibody (Cat. no. 553999), followed by Streptavidin-PE (Cat. no. 554061).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.