BD, 560083, BD™ Cytometric Bead Array (CBA) Human LT-α Flex Set

Assay Range: 10-2,500 pg/mL
Reactivity: Human (QC Testing)
Regulatory Status: RUO
RRID: AB_2869300
Description: Description The BD™ CBA Human LT-α Flex Set is a bead-based immunoassay capable of measuring human lymphotoxin-α (LT-α), formerly known as tumor necrosis factor-β, in serum, plasma, and cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human LT-α Flex Set. The biology and function of LT-α has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).
Preparation And Storage: Preparation And Storage This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 2,500 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
Recommended Assay Procedures: Recommended Assay Procedures The BD CBA Human LT-α Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human LT-α Flex Set will be 2,500 pg/ml. An example standard curve is shown in Figure 1. When multiplexing the BD CBA Human LT-α Flex Set assay with the BD CBA Human IL-6 Flex Set assay (Cat. No. 558276) or the BD CBA Human Fas Ligand Flex Set assay (Cat. No. 558330), significantly higher background will be seen in the human IL-6 or Fas Ligand assays. This increased background, while reducing the sensitivity of the human IL-6 or Fas Ligand assay, will not effect the quantitation of human LT-α, Fas Ligand or IL-6 in any other way. The BD CBA Human LT-α Flex Set cannot be used in the same assay well with the following BD CBA Human Soluble Protein Flex Set reagents: Flex Set Bead Position Catalog Number BD CBA Human IL-3 Flex Set D5 558335 BD CBA Human VEGF Flex Set B8 558336 BD CBA Human OSM Flex Set D5 560084 The BD CBA Human LT-α Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Human Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/flexset. Performance Limit of Detection: The theoretical limit of detection is 0.2 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n =30) + 2 standard deviations.
Product Notices: Product Notices Source of all serum proteins is from USDA inspected abattoirs located in the United States. ProClin is a trademark of Rohm and Haas Company. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
Description : Quantity/Size : Part Number : EntrezGene ID
N/A : 100.0 : N/A : N/A