BD, 560091, BD Phosflow™ Alexa Fluor® 647 Mouse anti-PDPK1 (pS241)

Alternative Name: PDK1 (pS241), PKB Kinase (pS241)
Reactivity: Human (QC Testing), Mouse, Rat (Predicted)
Isotype: Mouse IgG1, κ
Immunogen: Phosphorylated Human PDPK1 Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_1645432
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures This mAb was characterized by flow cytometry (Flow) and western blot analysis (WB) using these model systems: Method Species Cells Treatment Fixation Perm buffer Result Flow Human Jurkat Calyculin A + Okadaic Acid Lyse/Fix or Cytofix III Up-regulation Flow Human Jurkat Calyculin A + Okadaic Acid Lyse/Fix I or II Unsatisfactory Flow Human PBMC Calyculin A + Okadaic Acid Lyse/Fix III Up-regulation WB Human Jurkat Calyculin A + Okadaic Acid 63 kDa