BD, 561059, BD Pharmingen™ APC Rat Anti-Mouse IL-10

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG2b
Immunogen: Recombinant mouse IL-10
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398558
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining for Flow Cytometric Analysis: The APC-conjugated JES5-16E3 antibody can be used for multicolor flow cytometric analyses to identify and enumerate IL-10 producing cells within mixed cell populations (see image). For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells).  For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or  Chapter 4: Immunofluorescent Staining of Intracellular Molecules for Flow Cytometric Analysis in the Techniques for Immune Function Analysis Application Handbook 1st Edition. 2003 . ELISA: The purified JES5-16E3 is useful as a capture antibody for a sandwich ELISA for measuring mouse IL-10 protein levels.