Bio-Rad, 1725274, SsoAdvanced Universal SYBR ® Green Supermix, 2,500 x 20 µl rxns, 25 ml (5 x 5 ml)

25 ml (5 x 5 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl 2 , SYBR ® Green I, ROX normalization dyes, for 2,500 x 20 µl reactions
Overview
SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d , stabilizes the polymerase-template complex, providing superior inhibitor tolerance , increased processivity , specificity, and greater speed without affecting PCR sensitivity , efficiency, or reproducibility.
SsoAdvanced Universal SYBR Green Supermix lets you:
• Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
• Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
• Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
• Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
• Obtain better results with Bio-Rad's PrimePCR assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance
Instrument Compatibility
SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
• StepOne/StepOnePlus
• 7500, ViiA 7
• 7000, 7300, 7700, 7900HT, QuantStudio Systems
• Mx3000P, 3005P, 4000
• Mastercycler ep realplex 2, 4
• Corbett Rotor-Gene 3000, 6000, Q
• LightCycler 480, 96
• LightCycler 1.0, 1.5, 2.0*
• LightScanner HR-1
• LightScanner 32*
* BSA must be added according to instrument specifications
Applications and Uses of SsoAdvanced Universal SYBR Green Supermix
• qPCR/real-time PCR
• Gene expression analysis
• Pathway analysis
• Absolute quantification
• Chromatin immunoprecipitation (ChIP) qPCR
• Mutation detection
• Pathogen detection
• Viral detection (load)
• Characterization of genetically modified organisms (GMO)
• Genetic profiling
Packaging Options
Related Products
We have developed SsoAdvanced universal probes supermix , which is available in several formats (200–5,000 reactions).
Bio-Rad offers a complete workflow solution for real-time PCR, including:
• RNA isolation kits
• Reverse transcription kits
• Real-time PCR supermixes
More Information
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols . We also provide insights into analysis of qPCR data and troubleshooting reactions .
* U.S. patent 7,560,260.