Qiagen, 337011, qBiomarker Somatic Mutation PCR Assays

PCR assay and master mix

Features

- Simple real-time PCR procedure
- High sensitivity and wide dynamic range
- Designed for routine use on most qPCR instruments
- Master mix is included

Product Details

qBiomarker Somatic Mutation PCR Assays identify the presence of individual specific sequence mutations present in cell lines or research samples that are critical for toxicological, drug development, and cancer studies. The mutations are selected from comprehensive curated databases (e.g., COSMIC) and literature reviews based on their clinical or functional relevance and frequency in patient populations.

Procedure

After the isolation of genomic DNA from fresh, frozen, or fixed samples, add an aliquot of each sample to a separate real-time PCR tube containing the appropriate master mix (included) and the qBiomarker Somatic Mutation PCR Assay. Then, add an aliquot of each sample to a separate real-time PCR tube containing the appropriate master mix (included) and the corresponding reference gene copy assay. Finally, run the recommended cycling program.

Determine the C T values for each mutation-specific assay and the corresponding reference gene copy assay for each sample using your instrument's software. Then, paste the values into the correct Excel-based data analysis template to determine which samples contain the tested mutation.

Applications

qBiomarker Somatic Mutation PCR Assays are highly suited for the rapid and accurate identification of individual specific sequence mutations present in fresh, frozen, or fixed samples.