Qiagen, 59695, EpiTect PCR Control DNA Set (100)

Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs

Features

- Convenient, ready-to-use, quality-controlled DNA
- Bisulfite-converted DNA for control experiments
- Suitable for all methylation analyses

Procedure

Standardized workflows in epigenetics

Accessing epigenetic information is of prime importance for many areas of biological and medical research — particularly oncology, but also stem cell research and developmental biology. However, the analysis of changes in DNA methylation is challenging, due to the lack of standardized methods for providing reproducible data particularly from limited sample material. With its newly introduced EpiTect solutions, QIAGEN makes available standardized, pre-analytical and analytical solutions from DNA sample collection, stabilization and purification, to bisulfite conversion and real-time or end-point PCR methylation analysis or sequencing (see figure " Standardized workflows in epigenetics ").

Applications

- Evaluating primer specificity for MSP
- Quantification standard for HRM and MethyLight PCR
- Evaluating primer and probe specificity for MethyLight PCR
- Checking efficiency of bisulfite conversion reactions

EpiTect Control DNAs are suitable for use in control reactions for all types of methylation analyses, including: