Qiagen, 675013, ipsogen RUNX1-RUNX1T1 Kit (24)

For 24 reactions: ABL Control Gene Standards, RUNX1-RUNX1T1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix RUNX1-RUNX1T1 Fusion Gene

Features

- Sensitive quantitative assay
- Accurate quantification relative to ABL control gene expression
- EAC standardized assay for reproducible results
- Optimal quality control and ISO 13485 certification
- Easy workflow with ready-to-use solutions

Performance

To ensure highest sensitivity, the ipsogen RUNX1-RUNX1T1 Kit has been optimized to detect ABL and RUNX1-RUNX1T1 fusion gene transcripts over a wide dynamic range (see figure " Sensitive detection of RUNX1-RUNX1T1 and ABL standards ").

Principle

The ipsogen RUNX1-RUNX1T1 Kit is a ready-to-use kit for detection and quantification of RUNX1-RUNX1T1 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific RUNX1-RUNX1T1 fusion gene transcripts, relative to ABL control gene expression in total RNA (see figures " RUNX1-RUNX1T1 fusion gene transcript " and " ABL control gene transcript ", and provides high levels of specificity, sensitivity, and reproducibility. Each ipsogen RUNX1-RUNX1T1 Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for the RUNX1-RUNX1T1 fusion gene. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations.