Thermo Fisher Buffers & Standards for pH, Conductivity, HPLC/IC/LC-MS

This collection brings together Thermo Fisher buffer solutions and certified standards you rely on across cell culture, protein work, electrochemistry, chromatography, and mass spectrometry. Browse ready-to-use PBS/Tris/HEPES, pH and conductivity standards, LC/IC reference mixes, and LC-MS calibration solutions. As a distributor, Iright can supply a wide range of Thermo Fisher brands (Gibco, Thermo Scientific Chemicals, Orion, Pierce, Dionex) and provide quick COA/SDS on request.

Ready-to-Use pH Buffers (PBS, Tris, HEPES) for Consistent Results

Stable pH and ionic strength are the backbone of reproducible methods. In this section, you’ll quickly pinpoint the right buffer family and grade—cell-culture PBS, molecular-biology Tris-HCl, or zwitterionic HEPES—then decide on concentration, sterility, and packaging that fit your workflow and documentation needs.

At-a-glance buffer families

Calibration & Reference Standards for HPLC/IC/LC-MS and pH/Conductivity

Quantitation and daily QC depend on traceable references. Below you’ll find electrochemistry standards for pH and conductivity, IC multi-anion standards for retention/response checks, and LC-MS calibration solutions spanning positive/negative modes and extended mass ranges.

What you’ll use and when

• pH buffers (NIST-traceable) to bracket and confirm meter accuracy at 4.01/7.00/10.01 (and beyond 12.46 for strong alkaline checks).
  
• Conductivity standards at 100 µS/cm, 1413 µS/cm, 12.9 mS/cm, 111.9 mS/cm for routine calibration or field verification.
  
• IC combined anion standards (e.g., Seven Anion Standard I/II) to validate separation and response in routine anion determinations.
  
• LC-MS calibration solutions (Orbitrap/ion trap/triple quad; positive/negative/extended range) to maintain mass accuracy and sensitivity with ready-to-use mixes.

How to Choose: Application-Based Selection of Buffers and Standards

Starting from the job at hand speeds selection. Use the matrix below to match your task to the right product type and constraints (sterility, volatility for MS, traceability for audits).

Representative SKUs & Specs (Thermo Fisher Catalog Numbers)

The list below highlights commonly requested items across buffer and standard categories so you can compare spec, volume, and brand line at a glance.

Storage, Handling & MS Compatibility Notes

Getting storage right protects stability and your results. Most ready-to-use buffers ship at ambient and store at room temperature or 2–8 °C; avoid contamination by aliquoting, using clean tips, and capping promptly.

For LC-MS work, prefer volatile salts/buffers (e.g., ammonium formate/acetate) in the method itself and keep non-volatile buffers for offline steps. (For PBS cell-culture packs, check sterile-filtered status and Ca/Mg content in the spec table or COA.)

Quick reminders

• Do not freeze solutions unless the datasheet allows it; repeated freeze-thaw degrades performance.
• For pH buffers, record temperature—calibration tables specify pH vs. temperature tolerance.
  
• LC-MS calibration solutions are ready to use; follow the instrument family guidance (positive/negative mode, extended range) and store per label.

Methods & Typical Workflows (Short Notes)

Daily routines are short and repeatable. Use these quick patterns to map products to tasks and keep your documentation clean.

• Cell-culture wash/transfer → Gibco PBS 1× (no Ca/Mg) → rinse/transport → log lot/expiry → attach COA in your batch record.
  
• pH meter daily check → Orion 7.00 then 4.01 (or 10.01) → verify slope and offset → record temperature and lot.
  
• Conductivity meter verification → 1413 µS/cm and 12.9 mS/cm standards → confirm within tolerance → document.
  
• LC-MS mass calibration → choose LTQ/Triple Quad/FlexMix per instrument → run positive/negative as required → archive calibration report and solution lot.
  
• IC system suitability → Dionex Seven Anion Standard II → check retention times/peak areas → adjust eluent as needed.

FAQs: Buffers, pH Standards, Conductivity & LC-MS Calibration

A brief set of answers helps you move from shortlist to purchase without detours. If you need a formal quotation or documentation packet, contact Iright anytime.

Q1. Do I need Ca/Mg in PBS for cell handling?

Generally no—choose PBS without Ca/Mg for washing and transfers to avoid unwanted adhesion; confirm sterile-filtered status for direct use in culture.

Q2. Which pH buffers should I keep on hand?

Most labs maintain 4.01, 7.00, and 10.01 buffers; alkaline labs may add 12.46. Pick NIST-traceable bottles or 5×60 mL packs for field use.

Q3. What conductivity values cover routine checks?

100 µS/cm, 1413 µS/cm, 12.9 mS/cm, and 111.9 mS/cm span low to high ionic strengths for most meters and methods.

Q4. How do I choose between LTQ/Triple Quad/FlexMix LC-MS solutions?

Match the solution to your instrument family and mode: LTQ positive, Triple Quad (TSQ), or FlexMix for positive/negative with extended ranges on Orbitrap platforms.

Q5. Are IC standards traceable?

Dionex combined anion standards are designed for routine determinations and supplied with COA; select size and formulation that match your method.

Request a Quote from Iright — Fast COA/SDS Access & Availability

Tell us the catalog numbers, volumes, and any certificate requirements (e.g., NIST traceability, endotoxin limits). Iright will confirm stock, lead time, and documentation, and can help you standardize a short, auditable list of buffers and standards for your lab. (If you prefer equivalents under the same brand line, we can suggest them side by side.)