Thermo Fisher, 90106, Pierce™ S-Nitrosylation Western Blot Kit and HENS Buffer

Pierce™ S-Nitrosylation Western Blot Kit and HENS Buffer The Pierce S-Nitrosylation Western Blot Kit provides cell lysis/reaction buffer, sulfhydryl-reactive blocking agent, reducing agent, labeling agent, and anti-TMT antibody to label 40 samples (100 μg each). As with traditional S-nitrosylation switch assays, unmodified cysteines are blocked by a sulfhydryl-reactive compound. S-nitrosylated cysteines are selectively reduced with ascorbate in HENS Buffer for labeling with iodoTMTzero reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT reagent-modified proteins is facilitated using an anti-TMT antibody.
Description: HENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit
Reactivity: All species
Detection Method: S-Nitrosylation
Product Line: Pierce™
Quantity: 100 mL
Unit Size: Each