BD, 551413, BD Pharmingen™ Purified Rat Anti-Human CD184

Alternative Name: CXCR4, Fusin
Reactivity: Human (QC Testing)
Isotype: Rat IgG2a, κ
Immunogen: Human CXCR4 fusion protein
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394191
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures The purified 1D9 antibody can be used for the immunofluorescent staining and flow cytometric analyses of human leukocytes and cell lines that express CXCR4 (see figure). A multistep step staining procedure is recommended to amplify immunofluorescent signals for the flow cytometric analysis of human CXCR4 expression: Step 1:   Incubate 10e6 cells with 0.06 - 0.5 µg of purified 1D9 antibody at 4°C for 15 - 20 minutes. Wash cells two times with staining medium containing sodium azide (e.g., Dulbecco's PBS or tissue culture medium [without phenol red and biotin] with 0.09% sodium azide and 2% heat-inactivated FCS or 0.2% BSA). Step 2:   Incubate the cells with biotinylated mouse anti-rat IgG2a (Cat. No. 553894) at 4°C for 20 minutes. Wash cells two times. Step 3:   Incubate the cells with ≤0.06 µg of streptavidin-phycoerythrin (Cat. No. 554061) at 4°C for 20 minutes. Wash two times. Resuspend cells in staining medium and analyze stained cells by flow cytometry.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.