BD, 553621, BD Pharmingen™ Purified Rat Anti-Mouse I-A/I-E

Alternative Name: H-2I; I-Ad, -Ed; I-Eb and I-Ek MHC class II alloantigens; Ia Ag; MHC II
Reactivity: Mouse (QC Testing)
Isotype: Rat DA, also known as DA/HA IgG2a, κ
Immunogen: BALB/c mouse epidermal Langerhans cells
Application: Flow cytometry (Routinely Tested), Immunoprecipitation, Intracellular staining (flow cytometry) (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394956
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Other reported applications include immunoprecipitation and intracellular immunofluorescent staining.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.