BD, 553893, BD Pharmingen™ Purified Mouse Anti-Rat IgG2a

Reactivity: Rat (QC Testing)
Isotype: Mouse SJL IgG2b, κ
Immunogen: Rat Pooled IgG
Application: ELISA (Routinely Tested), Flow cytometry (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_395121
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Purified Mouse Anti-Rat IgG2a (Cat. No. 553893) is routinely tested by ELISA for its ability to recognize and bind Rat IgG2a, kappa (clone R35-95) and Rat IgG2a, lambda (Clone B39-4) while not binding all other Rat Ig isotypes.  Biotin Rat Anti-Mouse IgG2b (clone R12-3; Cat. No. 553393) along with a streptavidin reporting system is used for detection. For sandwich Rat IgG2a ELISA, Biotin Mouse Anti-Rat IgG2a (Cat. No. 553894) is optimal for detection with Purified Mouse Anti-Rat IgG2a (B46-7; Cat. No. 553918) for capture. RG7/1.30 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG2a, we recommend FITC Mouse Anti-Rat IgG2a (Cat. No. 553896).
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.