BD, 554499, BD Pharmingen™ Biotin Anti-Human and Viral IL-10

Reactivity: Human, Viral (QC Testing)
Isotype: Rat IgG2a
Immunogen: Human IL-10
Application: ELISA Detection (Routinely Tested), Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395435
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture (Human/Viral IL-10): Biotinylated- JES3-12G8 (Cat. No. 554599) is useful as an ELISA capture antibody when used in combination with purified JES3-9D7 (Cat No. 554497) and with recombinant human IL-10 protein standard (Cat. No. 554611). Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23 , SCF, TNF, LT-α, VEGF; rat IL-2, IL4, IL-6, IL-10, GM-CSF, IFN-γ, TNF). Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISAreagents are not recommended for assaying serum samples. For testing human IL-10 in complex biological fluids like serum or plasma, our human IL-10 OptEIA™ ELISA Set (Cat. No. 555157) and OptEIA™ ELISA Kit (Cat. No. 550613) are recommended. WB: The JES3-12G8 antibody has been reported to be useful for Western blotting.. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.