BD, 554577, BD Pharmingen™ Recombinant Mouse IL-1β

Preparation And Storage: Preparation And Storage Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5-10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
Recommended Assay Procedures: Recommended Assay Procedures 1.  Biological Activity :  As measured by proliferation of TF-1 indicator cells at 10^5 cells/ml Specific Activity :  0.06 - 2.5 x10^8 Unit*/mg ED50 : 0.04-1.5 ng/ml; Observed dose response relationship: >50 fold *Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Investigators are advised to determine optimal concentrations for individual applications. 2. ELISA Standard :. Recombinant mouse IL-1β is useful as a quantitative standard for measuring mouse IL-1β  protein levels in an IL-1β -specific sandwich ELISA. For specific methodology, please visit the online protocols under "ELISA and ELISPOT" at our website, http://www.bdbiosciences.com/us/s/resources. To measure mouse IL-1β  in plasma or serum, we recommend the BD OptEIA™ Mouse IL-1β  ELISA Set (Cat. No. 559603), which is specially formulated for assay of cytokine in complex fluids.