BD, 555057, BD Pharmingen™ FITC Mouse IgG2b, κ Isotype Control

Alternative Name: Anti-DNS; Anti-Dansyl; IgG2b kappa; Isotype Control (anti-Dansyl)
Isotype: Mouse C.SW IgG2b, κ
Immunogen: Dansyl (DNS) Hapten
Application: Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395677
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometic Analysis: The PE-and FITC-conjugated 27-35 immunoglobulin (Cat. No. 555057, 555058 respectively) are suitable mouse IgG2b isotype controls for assessing the level of background staining on paraformaldehyde fixed/saponin-permeabilized rat or human cells for flow cytometric analysis. Use at comparable concentrations to antibody of interest (e.g., ≤ 0.5 µg mAb/1 million cells) (see image, middle panel). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology, please visit our web site, www.bdbiosciences.com/us/s/resources and refer to the protocols section under Intracellular Flow.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.