BD, 559043, BD Pharmingen™ Purified Mouse Anti-Human CD56

Alternative Name: NCAM1; NCAM-1; NCAM; Leu-19; Neural cell adhesion molecule 1; NKH1; MSK39
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2b, κ
Immunogen: Immunoaffinity-enriched adult human brain NCAM
Application: Flow cytometry (Routinely Tested), ELISA, Functional assay, Immunohistochemistry-frozen, Immunoprecipitation (Reported)
Concentration: 1.0 mg/ml
Workshop Number: V NK60
RRID: AB_397180
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Functional Studies: N-CAM is expressed on many cell and tissue types including neural tissue, developing and diseased smooth and cardiac muscle, NK lymphocytes, and a small percentage of T lymphocytes. Anti-N-CAM-16 monoclonal antibody (clone NCAM16.2) can be used with other anti-cell adhesion molecule monoclonal antibodies to better understand the role of N-CAM in nervous system inflammation and tumor cell metastasis. This antibody has been used with monoclonal antibodies to integrins αL, α4, β1, and β2, ICAM-1, VCAM-1 and E-selectin, and other monoclonal antibodies to N-CAM to study mononuclear leukocyte adhesion to neuroblastoma and cortical neural cells. NK lymphocytes and the subset of T lymphocytes that express N-CAM are unique in their ability to mediate direct, non-major histocompatibility complex-restricted cytotoxicity against certain tumor cells. Immunoprecipitation: Anti-N-CAM-16 monoclonal antibody (clone NCAM16.2) immunoprecipitates N-CAM from human brain, KG-1a cells, leukemia cells, and N-CAM-expressing neuroblastoma cells. Immunohistology: Anti-N-CAM-16 monoclonal antibody (clone NCAM16.2) can be used for staining of frozen or paraffin-embedded sections of human or rat neural and skeletal muscle tissues by indirect immunofluoresscence or immunoperoxidase metods, or staining of cultured LAN-1 human neuroblastoma cells by indirect immunofluorescence. Enzyme-linked immunosorbent assay (ELISA)/Radioimmunoassay (RIA): Anti-N-CAM-16 monoclonal antibody (clone NCAM16.2) can be used in an ELISA to detect N-CAM in brain extracts.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.