BD, 562341, BD Pharmingen™ APC Mouse anti-Human GARP

Alternative Name: LRRC32; Garpin; Glycoprotein A repetitions predominant
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Recombinant Human LRRC32
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_10926211
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Recommended Assay Procedures: Recommended Assay Procedures Suggested Staining Procedure for APC anti-Human GARP Antibody: 1.  Harvest PBMC after stimulation (12 hours) with Purified NA/LE Mouse Anti-Human CD3 (Cat. No. 555329) and Purified NA/LE Mouse Anti-Human CD28 (Cat. No. 555725). 2.  Wash the cells twice with stain buffer (eg. BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656). 3.  Stain 1 X 10^6 cells either with the APC anti-Human GARP antibody (Cat. No. 562341) or with APC Mouse IgG2b, κ Isotype Control (Cat. No. 555745) for 30 minutes on ice, protected from light. 4.  Wash cells twice with stain buffer. 5.  Stain for FoxP3. Refer to the Technical Data Sheet of BD Horizon™ V450 Mouse Anti-Human FoxP3, Cat. No. 560459, for detailed FoxP3 staining protocol. In brief, a.  Add 2 ml of 1 X FoxP3 buffer A to the cell pellet. b.  Centrifuge and incubate in 0.5 ml of buffer C for 30 minutes. c.  Wash twice with stain buffer and stain with V450 Anti-Human FoxP3 antibody (Cat. No. 560459) for 30-45 minutes. d.  Wash twice with stain buffer and acquire on the Flow cyotmeter. Note: BD Pharmingen™ Human FoxP3 Buffer Set (Cat. No. 560098) is a suggested companion product.
Product Notices: Product Notices Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).