BD, 563238, BD Pharmingen™ PE Mouse Anti-Human NCAM-1 (CD56)

Alternative Name: N-CAM-1, Neural cell adhesion molecule 1; NKH1; MSK39; Leu-19
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Human NCAM1 Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested), Flow cytometry (Tested During Development)
Vol. Per Test: 5 µl
RRID: AB_2738087
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures The R19-760 antibody may be used for both intracellular and cell-surface flow cytometric applications.  For intracellular flow cytometry, various fixatives and permeabilization buffers are suggested (see Suggested Companion Products ).
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Brilliant Violet™ 421 is a trademark of Sirigen. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.