BRAND / VENDOR: Cytiva

Cytiva, 29707299, PrismA ELISA™ kit

CATALOG NUMBER: 29707299

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Product Description
PrismA ELISA kit The PrismA ELISA™ kit is an enzyme-linked immunosorbent assay designed to measure residual ligand leakage when using MabSelect PrismA™ chromatography resin and Fibro™ PrismA chromatography adsorbers. The kit is used during the development and manufacture of monoclonal antibody (mAb) biopharmaceuticals. • Matched PrismA protein A ligand included in the kit removes the need to source the standard separately and create a custom protocol • Parts per billion sensitivity minimizes the risk of undetected residual PrismA ligand • High IgG tolerance simplifies sample preparation • Consistently low intra- and inter-plate variability ensures reproducible data • All-in-one kit containing all reagents needed to perform the assay The PrismA ELISA™ kit contains all reagents required to perform the assay. This includes the PrismA ligand optimized for use in ELISA and polyclonal antibodies raised specifically against the PrismA ligand. The PrismA ELISA™ kit was developed with a focus on usability, robustness, and environmental sustainability. High IgG tolerance Downstream samples generally have high concentrations of IgG, which creates a challenge for protein A measurement. Accurate measurement requires dissociation of the ligand and IgG, which is especially tricky when the concentration of protein A ligand is very low (< 1 ppm) due to the large excess of IgG. To determine the effect of IgG on dissociation, we spiked high, medium, and low concentrations of PrismA ligand into decreasing concentrations of IgG. The PrismA ELISA™ kit could accurately determine PrismA concentration in samples containing as high as 20 mg/mL of IgG. The PrismA ELISA™ kit delivers high sensitivity for detecting trace amounts of ligand—even during the final stages of purification: Lower limit of detection (LLD): 10 pg/mL (+IgG) Lower limit of quantification (LLQ): 10 pg/mL (+IgG) View data file to see supporting data for these performance specifications. Perform with confidence across common buffer conditions The PrismA ELISA™ kit delivers accurate and reproducible results over multiple experiments, and has been tested with common buffers used in downstream purification of monoclonal antibodies. The results showed the kit was compatible with all five buffers tested, as indicated by all dilutions of each sample (8/8) being within the acceptance criteria of the assay, and the parallelism being below the target of 20%. Contents of the PrismA ELISA™ kit include:
Component Volume
PrismA ELISA™ Kit Assay Buffer (1X) 10 mL
PrismA ELISA™ Kit Detection Antibody Diluent (1X) 15 mL
PrismA ELISA™ Kit Wash Buffer (10X) 25 mL
PrismA ELISA™ Kit TMB (1X) 15 mL
PrismA ELISA™ Kit Dissociation Buffer (1X) 30 mL
PrismA ELISA™ Kit Stop Buffer (1X) 15 mL
PrismA ELISA™ Kit Standard 1 vial
PrismA ELISA™ Kit Detection Antibody 140 µL
PrismA ELISA™ Kit 96 Well Strip-Plate 1 plate
PrismA ELISA™ Kit Plate Seal 1 seal
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Collaboration

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