Merck, A1888-5G, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is water soluble chemical compound used as chromogenic substrate: •ABTS together with hydrogen peroxide for reaction with peroxidase enzymes (like HRP for example). •ABTS together with laccase or bilirubin oxidase. ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures. A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).††† ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive.