Qiagen, 27406, QuickLyse Miniprep Kit (250)

250 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)

Features

- Rapid one-step lysis procedure
- Reduced protocol steps and hands-on time
- Up to 15 µg DNA from 1.5 culture volumes obtained
- Ready-to-use plasmid DNA in just 9 minutes

Product Details

The QuickLyse Miniprep Kit provides a rapid spin-column-based method for isolating sequencing-grade plasmid DNA. Bacterial cell resuspension, lysis, and DNA binding are achieved in a single buffer, enabling fast DNA purification with minimal hands-on time.

Performance

The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultrafast preparation of up to 15 µg plasmid DNA. The quality of the DNA ensures robust performance in applications, such as sequencing, restriction analyses, and cloning (see figure " Reproducible yields ").

The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including sequencing (see figure " Long read-lengths in automated sequencing "). Up to 15 µg of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (see figure " High yields of high-quality plasmid DNA "). Isolated plasmid DNA has OD 260 /280 ratios of 1.7–1.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.

Principle

Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.

Applications

The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including automated sequencing.