Qiagen, 47154, AllPrep Fungal DNA/RNA/Protein Kit (50)

For the extraction of protein and nucleic acids from fungal cell cultures

Features

- Efficient solubilization of total nucleic acids and cellular proteins from bacterial and fungal samples
- Sequential DNA, RNA and protein extraction in less than 40 minutes without precipitation
- Ready-to-use, high-quality nucleic acids and proteins for downstream applications

Product Details

Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. The bead-beating method and spin column technology effectively extract nucleic acids and proteins in a streamlined process. DNA and RNA are ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution.

Procedure

Cultured bacterial and fungal cells are first lysed using glass bead tubes, and nucleic acids and proteins are solubilized and mixed with a binding solution. DNA binds to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. The final flow-through captures protein. Each of the individual spin columns is then washed and the immobilized analyte is eluted.