Qiagen, 951054, EZ1&2 DNA Blood 350 µl Kit (48)

For 48 preps: Reagent Cartridge (Blood 350 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)

Features

- Rapid purification of up to 12 µg DNA from whole blood
- Purification of up to 14 µg DNA from buffy coat using the EZ1&2 DNA Blood 350 µl Kit
- Processing of 200–350 µl whole blood samples
- Process up to 24 samples at the same time for easy intra-run comparability

Product Details

EZ1&2 DNA Blood Kits contain all required reagents and labware for rapid, automated purification of DNA from 200-350 μl whole blood samples or buffy coat samples using magnetic-particle technology. Reagents are supplied in prefilled and sealed reagent cartridges, which ensures speed and convenience in loading the instrument.

EZ1&2 DNA Blood Kits are automated with the EZ2 Connect (1-24 samples per run). These kits can also be used with the EZ1 Advanced XL (1–14 samples per run).

Performance

The automated procedure provides high repeatability (see figures "High-quality DNA from whole blood" and "Reproducible yields of high-quality DNA from whole blood" ) and linear yields over a range of starting amounts. DNA purified using EZ1&2 DNA Blood Kits gives high-performance results in downstream applications, such as PCR (see figure "High-quality DNA for sensitive and specific analyses" ).

Principle

EZ1&2 DNA Blood Kit: EZ1 DNA Card
EZ1&2 DNA Blood 200 µl Kit: EZ1 Advanced XL DNA Blood Card
EZ1&2 DNA Blood 350 µl Kit: EZ1 Advanced XL DNA Blood Card EZ1 Advanced XL DNA Buffy Coat Card

Automated purification with EZ1&2 DNA Blood Kits requires use of either EZ2 Connect instruments or EZ1 instruments and EZ1 DNA Cards (see table “EZ1&2 DNA Blood Kits and EZ1 DNA Cards”).

EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

EZ1&2 DNA Blood Kits and EZ1 DNA Cards

Procedure

Whole blood samples (200 µl or 350 µl) are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1&2 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.

Applications

- Population genetics and pharmacogenomic research
- PCR and real-time PCR
- Sequencing
- Oncology research
- Genetic identity testing and forensics
- Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies

The high-quality blood obtained using EZ1&2 DNA Kits is suited for use in many applications, such as: