Qiagen, RP905A, TaqNova HS DNA Polymerase (500 U)

TaqNova HS DNA Polymerase, 10x TaqNova HS Reaction Buffer, 50 mM MgCl2 Storage temperature: -20°C

Features

- Catalyzes DNA synthesis in a 5’ → 3’ direction
- Shows no 3′ → 5’ exonuclease activity but has a 5’ → 3’ exonuclease activity
- Enables easy setup of a hot-start PCR reaction at room temperature
- Minimizes amplification of non-specific products and primer-dimers
- Amplifies fragments of up to 5 kb and leaves ´A´ overhangs

Product Details

TaqNova HS DNA Polymerase is a mixture of of recombinant thermostable Taq DNA polymerase from Thermus aquaticus and a highly specific monoclonal antibody, which acts as an inhibitor of the polymerization activity. The TaqNovaHS enables easy setup of a hot-start PCR reaction at room temperature.

It is recommended for a wide range of demanding applications that require highly specific amplification. The TaqNovaHS polymerase is a universal and easy-to-use DNA polymerase that works rapidly and effectively in various PCR conditions. The enzyme catalyzes DNA synthesis in a 5’ → 3’ direction, shows no 3′ → 5’ exonuclease activity, but has a 5’ → 3’ exonuclease activity.

It is supplied with 20 mM Tris-HCl (pH 8.0, 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol.

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μL reaction.

Performance

Assay: Specification
Functional activity: Passed
DNase contamination: None detected

Principle

The antibody binds reversibly to the enzyme, inhibiting polymerase activity at ambient temperatures, which prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The antibody is released from the polymerase during normal cycling conditions. The use of the TaqNovaHS Polymerase does not require any additional incubation step to activate the enzyme.

Procedure

Quality Control

DNase activity and DNase contamination were evaluated by incubating 1 μg of DNA with 10 units of the enzyme for 4 hours at 37°C. Results are visualized on an ethidium bromide-stained agarose gel. The product was extensively tested in PCR reaction.

Applications

- Hot-start and real-time PCR
- Singleplex and multiplex PCR
- PCR with DNA from various kinds of specimen
- Specific amplification of difficult templates (GC-rich)

This is used for applications such as: