Waters, 176001913, ACQUITY UPLC BEH Amide 1.7µm3.0x150 3-PK

ACQUITY BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology the ACQUITY Amide columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 12 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 3 mm
Length: 150 mm
Carbon Load: 12 %
eCord: Yes
UNSPSC: 41115709
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 3 pk

Additional Information
ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 3 mm X 150 mm, 3/pk Add the ACQUITY UPLC BEH Amide Column to your lab equipment collection in order to achieve outstanding retention of polar analytes across a wide range in polarity (including carbohydrates and sugars), structural moiety, and pka. As it has been designed to retain polar analytes and metabolites that are too polar to retain reversed-phase (RP) chromatography, the column enables the use of a wide operational range of mobile phase pH (2-11). The combination of BEH technology with a tri functionally-bonded amide phase in the column provides exceptional column lifetime and improves assay robustness. Make use of the chemically stable, tri functionally-bonded amide phase that is based on Waters’ novel ethylene bridged hybrid (BEH) particle technology by opting for the ACQUITY UPLC BEH Amide Column. This gives a new dimension in stability and versatility for HILIC (Hydrophilic Interaction Chromatography) separations. The use of unbonded BEH particles in the lab equipment also provides orthogonal separation mode for mixtures of polar and ionizable compounds. The column has been optimized and tested to produce efficient, reproducible separations under UPLC HILIC conditions, using a specific design that helps to overcome a major weakness of the HILIC stationary phases, chemical stability. As the ACQUITY UPLC BEH Amide Column provides increased mass spectrometry response, direct compatibility with sample preparation elutes (PPT, LLE, and SPE) as well as orthogonal selectivity compared to reversed-phase materials, it proves an excellent choice for lab equipment. Use the ACQUITY UPLC BEH Amide Column to conduct exceptional analysis of carbohydrates. The column provides many benefits, such as its 1.7 µm particle size enables high speed and high-resolution analysis in complex matrices while improving or maintaining chromatographic resolution. It also improves quantitation accuracy as it is not susceptible to Schiff base formation, unlike the amine-based columns. The increased pH stability demonstrated by the ACQUITY UPLC BEH Amide Column enables the use of high pH and high temperature to collapse, reducing sugar anomers, shortening analysis times, and improving MS detection. See the complete available range of Waters ACQUITY UPLC BEH Amide columns and shop for lab equipment such as the ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk directly from us.

FAQ
What Temperature Is Suitable For ACQUITY UPLC BEH Amide Column? The column can tolerate an operating temperature range between 20–90 ˚C.