BD, 553172, BD Pharmingen™ PE Hamster Anti-Mouse TCR β Chain

Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG2, λ1
Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10
Application: Flow cytometry (Routinely Tested), Fluorescence microscopy (Reported)
Concentration: 0.2 mg/ml
RRID: AB_394684
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2 to 4 hours prior to staining enhances the ability of anti-CD3e and anti-TCR β chain mAbs to detect the T cell receptor on immature thymocytes. This antibody conjugate is compatible with intracellular staining protocols using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714).