BD, 559047, BD Pharmingen™ Purified Mouse Anti-Human CD54

Alternative Name: Human ICAM-1, HICAM-1; BB2; P3.58
Reactivity: Human (QC Testing)
Isotype: Mouse IgG2b, κ
Immunogen: Major histocompatibility Class II antigen negative variant of human B-lymphoblastoid cell line
Application: Flow cytometry (Routinely Tested), Functional assay, Immunohistochemistry, Immunoprecipitation (Reported)
Concentration: 1.0 mg/ml
Workshop Number: IV B50, M165
RRID: AB_397183
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Functional Studies: ICAM-1 is a ligand for both the leukocyte function-associated antigen-1 (LFA-1) and Mac-1, and thereby mediates the adhesion of leukocytes to endothelial cells. Leukocyte binding to endothelial cells is a critical process in inflammatory responses such as rheumatoid arthritis, adult respiratory distress syndrome, chronic dematoses, and reperfusion myocardial injury. Anti-ICAM-1 monoclonal antibody (clone LB-2) inhibits phorbol ester-enhanced aggregation of EBV-B or lectin-activated normal blood mononuclear cells. Anti-ICAM-1 (clone LB-2) is specific for a functional epitope on ICAM-1 and inhibits LFA-1-ICAM-1 interactions. Immunoprecipitation: Under reducing conditions, anti-ICAM-1 monoclonal antibody (clone LB-2) immunoprecipitates a glycoprotein from EBV-immortalized B lymphocytes (EBV-B) with a molecular mass of 84 kDa by gel electrophoresis, while under nonreducing conditions a molecular mass of 77 kDa was observed. Immunohistology: Anti-ICAM-1 monoclonal antibody (clone LB-2) can be used for immunohistochemistry staining.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.