Bio-Rad, 1610398, Precision Plus Protein™ WesternC™ Blotting Standards, value pack, 250 applications

1.25 ml (5 x 250 µl) Precision Plus Protein WesternC Standards and 625 µl (5 x 125 µl) StrepTactin-HRP conjugate, 250 applications
Overview
Free Protein Standards Selection Magnet
Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple.
Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These standards contain ten Strep -tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep -tagged recombinant proteins for molecular weight sizing after blot development.
Product Contents
5 - Precision Plus Protein WesternC Standards, 250 μl ( 1610376 )
5 - Precision Protein™ StrepTactin-HRP Conjugate, 125 μl ( 1610381 )
Applications and Uses
• Monitoring electrophoretic separation
• Monitoring blot transfer efficiency with easy orientation via multicolor standards
• Molecular weight sizing of proteins after blot development
• Fluorescent blot detection of prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range
More Information
Loading buffer: 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN 3 , 0.01% bromophenol blue.
Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection.
Packaging Options
Related Products
• Precision Protein StrepTactin-HRP Conjugate, 300 µl ( 1610380 )
• Precision Protein StrepTactin-HRP Conjugate, 125 µl ( 1610381 )
• StrepTactin-AP Conjugate, 300 µl ( 1610382 )
More Precision Plus Protein Standards:
• Precision Plus Protein Unstained Standards
• Precision Plus Protein Prestained Standards
• IEF and 2-D Electrophoresis Protein Standards
Related Categories
• Protein Ladders and Standards
• Precast Protein Gels
• Protein Electrophoresis and Blotting
• Western Blotting
Supporting Documents
• Precision Plus Protein WesternC Standards Instruction Manual
• StrepTactin HRP-Conjugate and AP-Conjugate Instruction Manual
Online Resources
• Protein Electrophoresis
• Western Blotting