Qiagen, 260001, QIAcuityDx Nanoplate 26k 24-well (10)

10 QIAcuityDx Nanoplate 26k 24-well, 11 QIAcuity Nanoplate Seals

Features

- QIAcuityDx Nanoplate 26k 24-well
- Majority of QIAcuity life science configurations supported in Utility mode
- Up to 8500 or 26,000 partitions per well
- SBS format

Product Details

Product: Cat. no.
QIAcuityDx Nanoplate 26k 24-well: 260001
QIAcuity Nanoplate 26k 24-well: 250001
QIAcuity Nanoplate 8.5k 24-well: 250011
QIAcuity Nanoplate 8.5k 96-well: 250021
Nanoplate Seals*: 250099
Nanoplate Tray: 250098
QIAcuity Nanoplate Adapter: 9027242

Nanoplates are microfluidic digital PCR plates that enable 24 or 96 samples to be run with up to 8500 or 26,000 partitions per well. All four nanoplates are designed to run on QIAcuityDx Four instruments.

In addition, QIAcuity life science configurations across 24 or 96 reactions and varying partitions between 8.5k and 26k may be used within the Utility mode of QIAcuityDx. Note : The IVD mode only allows use of QIAcuityDx plates.

The different nanoplate configurations can only be used within the QIAcuity dPCR instrument family. In the Utility mode, use the dedicated QIAcuity Nanoplate Adapter when performing automated liquid handling and PCR setup in a QIAcuity nanoplate using the QIAgility. Once done, load the plate onto the QIAcuityDx Four instrument for the dPCR reaction.

Would you like to learn more about the product and be contacted by one of our dPCR specialists? Contact us for more information and we will get in touch with you shortly.

Use of QIAcuityDx Nanoplates and Accessories in Utility and IVD mode

*Nanoplate Seals are for use with QIAcuity Nanoplates only.

Performance

Type: Frame color
QIAcuityDx Nanoplate 26k 24-well (IVD mode or Utility mode): Red
QIAcuity Nanoplate 26k 24-well (Utility mode): Blue
QIAcuity Nanoplate 8.5k 24-well (Utility mode): White
QIAcuity Nanoplate 8.5k 96-well (Utility mode): Gray

These are specially designed plates used for digital PCR reactions in QIAcuityDx instruments. We offer four nanoplate types, all in the Society for Biomolecular Screening (SBS) format, but with different specifications for different application needs.

Principle

In just 3 simple steps, you can get your dPCR results in approximately 2 hours: pipette and load, run the experiment, analyze results. The principle of the dPCR reaction in the nanoplates is described here .

Procedure

Just like in a qPCR workflow, sample preparation includes the transfer of master mix, probes and primers to a 24- or 96-well nanoplate followed by the addition of samples. The system integrates partitioning, thermocycling and imaging into a single, semi-automated instrument that achieves sample to result in approximately 2 hours. Analysis is performed using the Software Suite. This provides the concentration in copies per microliter of your target sequence when using the Utility mode and can also be used for quality control, such as for positive samples or No Template Control (NTC). This analysis can be extended to remote computers within the same local area network (LAN).

Applications

- Rare mutation detection
- Copy number variation analysis
- Gene expression analysis
- Pathogen detection
- Genotyping
- Cell and gene therapy
- Residual DNA quantification

QIAcuity Nanoplates, in combination with the QIAcuityDx Four and QIAcuityDx Universal MasterMix Kit, enable digital PCR applications, including: