Qiagen, 937266, QIAsymphony DNA Maxi Kit (96)

For 96 preps of 4 ml each: 2 reagent cartridges, 2 piercing lids, 2 reuse seal sets, buffers and Proteinase K solution

Features

- Process 24 samples in 4.75 hours
- Reduce risk of human error with prefilled reagent cartridges
- Automate tracking of samples and reagents using a bar code system
- Get highly intact DNA regardless of stabilization method

Product Details

Automate the purification of high-quality gDNA from human whole blood samples (up to 4 ml) using the QIAsymphony SP.

Performance

Automated DNA purification from 4 ml on the QIAsymphony SP results in high yields (measured via gel electrophoresis and spectrophotometer ) of high-molecular-weight DNA (see High-molecular-weight DNA ) in 4.75 hours (see QIAsymphony DNA Maxi Kit workflow ), and even faster for 2 ml or for fewer than 24 samples.

Principle

- The QIAsymphony SP takes 1 ml from the 4 ml sample and lyses that portion.
- While the first 1 ml is in the bind process, a second 1 ml is taken from the sample and gets lysed simultaneously.
- DNA from this 1 ml is bound with the same magnetic beads that were used for the first 1 ml. While this is happening, the third 1 ml is being lysed.
- DNA from the third 1 ml is bound in the same magnetic beads used with the previous portions. At the same time, the fourth 1 ml is being lysed.
- DNA from the last 1 ml portion is bound like the others.

The QIAsymphony DNA Maxi Kit uses an interlaced system (see QIAsymphony DNA Maxi Kit workflow ) that shortens the time needed to complete the process:

Procedure

The QIAsymphony SP performs all 4 steps (lyse, bind, wash and elute) of the purification procedure for up to 36 samples in a single run.