Qiagen, 955134, EZ1&2 Virus Mini Kit v2.0 (48)

For 48 preps: Reagent Cartridges (Virus Mini v2.0), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer AVE, carrier RNA

Features

- Simultaneous purification of viral DNA and RNA
- Higher analytical sensitivity, even with low viral titers
- Highly reproducible results
- Linear yields from a wide range of viral titers
- Prefilled, sealed reagent cartridges for easy handling

Product Details

The EZ1&2 Virus Mini Kit v2.0 provides a fully automated procedure for simultaneous purification of viral DNA and RNA from serum, plasma, CSF, urine, respiratory samples, and other cell-free body fluids for sensitive analytical detection in downstream assays. The kit provides optimized binding conditions for more robust and reproducible capture of nucleic acids, and improved wash conditions for higher analytical sensitivity in downstream assays.

The EZ1 DSP Virus Kit is also available for viral nucleic acid purification for in vitro diagnostics (not available in all countries).

The EZ1&2 Virus Mini Kit v2.0 is automated with the EZ2 Connect (1–24 samples per run). This kit can also be used with the EZ1 Advanced XL (1–14 samples per run).

Principle

- the EZ2 Connect
- the EZ1 Advanced XL and the EZ1 Advanced XL Virus Card v2.0

Automated purification with the EZ1&2 Virus Mini Kit v2.0 requires use of one of the following:

EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.

Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–24 samples (EZ2 Connect) or 1–14 samples (EZ1 Advanced XL) in a single run. Prefilled and sealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases.

Procedure

Serum or plasma samples (100 µl, 200 µl, or 400 µl) are loaded onto the instrument, and viral nucleic acids are purified in one step following the automated EZ1&2 Virus procedure. Viral nucleic acids are eluted in a volume of 150 µl, 120 µl, 90 µl or 60 µl and are ready to use in downstream applications.

The instrument makes viral nucleic acid purification convenient and reliable. The EZ1&2 Virus Mini Kit v2.0 provides prefilled, sealed reagent cartridges that help to prevent human error during setup of the instrument.