Qiagen, 959734, EZ2 RNA FFPE Kit (48)

For 48 preps: EZ2 RNA FFPE cartridge, disposable filter-tips and tip-holders, tubes, Paraffin Removal Solution, DNase Booster, RNase-free DNase I, RNase-free water

Features

- Recovers large amounts of amplifiable RNA
- Removes paraffin without using xylene or similar solvents
- Pretreatment-to-elution workflow is fully automated on the EZ2 Connect
- End product is highly suitable for downstream real-time RT-PCR, NGS or digital PCR (dPCR)

Product Details

The EZ2 RNA FFPE Kit purifies large amounts of amplifiable RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. This kit's protocol is fully automated from pretreatment to elution using the EZ2 Connect.

Principle

There are two ways that the EZ2 RNA FFPE Kit makes RNA extraction more efficient: First, it removes paraffin without multiple wash steps. This reduces the risk of losing sample material and the RNA they hold. Second, it fully automates all procedures after the deparaffinization step, so the number of tedious hands-on steps are radically reduced.

Procedure

To remove paraffin from the FFPE tissue sample, add Paraffin Removal Solution to the sample sections, vortex for 10 seconds, centrifuge briefly, then incubate for 3 minutes. Your manual procedure ends here.

Transfer the tubes containing the sample sections into the EZ2 Connect, set up and start the run (see “ Fully automated, from pretreatment to elution ”).

If you enabled QIAsphere connectivity in your EZ2 Connect, you will receive a notification on your desktop or mobile device when the run is finished.

Applications

The EZ2 RNA FFPE workflow is largely automated. All steps except deparaffinization actually happen during the EZ2 Connect run. When the run is over, the eluate that you take out of the instrument is suitable for downstream real-time RT-PCR, dPCR or NGS.