Thermo Fisher, 18068015, DNase I, Amplification Grade Invitrogen™

DNase I, Amplification Grade Invitrogen™ DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. It is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.
ApplicationRemoving DNA from RNA and protein preparations.
Specific activitySpecific activity is >10,000 units/mg.
SourcePurified from bovine pancreasPerformance and quality testingRibonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined.
Unit definitionOne unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.
001 A260units/min/mL of reaction mixture at 25°C.
Unit reaction conditions0.
1 M sodium acetate (pH 5.
0), 5 mM MgCl2, 50 μg/mL calf thymus DNA, and enzyme in 1 mL for 10 min at 25°C.
Compatible Buffer: Reaction Buffer
Quantity: 100 U
Shipping Condition: Approved for shipment on Wet or Dry Ice
Enzyme: DNase
Unit Size: Each