Thermo Fisher, E33651, EnzChek™ Ultra Amylase Assay Kit

EnzChek™ Ultra Amylase Assay Kit The EnzChek™ Ultra Amylase Assay Kit provides a solution-based assay featuring the speed, high sensitivity, and convenience required for measuring amylase activity or for screening amylase inhibitors in a high-throughput format. This EnzChek™ kit contains a starch derivative—the DQ™ starch substrate—that is labeled with BODIPY™ FL dye to such a degree that the fluorescence is quenched. This substrate is efficiently degraded by amylase; digestion relieves the quenching and yields highly fluorescent fragments. The accompanying increase in fluorescence is proportional to amylase activity and can be monitored with a fluorescence microplate reader or fluorometer, using standard fluorescein filters.
EnzChek™ Ultra Amylase Assay Kit Specifications:
• Label (Ex/Em): BODIPY™ FL conjugate (∼502/512 nm)
• Kit contains lyophilized substrate, 10X reaction buffer, substrate solvent, a fluorescent standard, and a detailed protocol
• Sufficient reagents are supplied for 500 assays (using a 100 μL assay volume in a 96-well microplate assay format)Find Fluorescent Substrates for Other GlycosidasesIn addition to the EnzChek™ Ultra Amylase Assay Kit, we offer kits and substrates to measure xylanase, lysozyme, β-galactosidase, and more. ReviewDetecting Glycosidases—Section 10.
2in the Molecular Probes™ Handbook for more information on these products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Detection Method: Fluorescence Intensity
Quantity: 1 Kit
Shipping Condition: Room Temperature
Substrate Properties: Molecular Substrate
Substrate Type: Amylase Substrate
Target Enzyme: Amylase
For Use With (Application): Ultra Amylase Assay
For Use With (Equipment): Fluorescence Microplate Reader
Product Line: EnzChek
Product Type: Amylase Assay
Unit Size: Each