Waters, 176003906, ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 150 mm, 1/pk and BEH125 SEC Protein Standard Mix

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 150 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note: This column includes the BEH125 SEC Protein Standard (PN: 186006519) at no additional cost.  Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications
Chemistry: SEC
Separation Mode: SEC
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 10000 psi (690 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 80000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 125 Å
QC Tested: Protein
Format: Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UPLC, UHPLC
Particle Technology: BEH
Technique: SEC
USP Classification: L33
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 12 %
UNSPSC: 41115709
Application: Protein
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 150 mm, 1/pk and BEH125 SEC Protein Standard Mix Add the ACQUITY UPLC Protein BEH SEC Column to your lab equipment collection as it is based upon the highly successful BEH particle technology and is the first commer­cially available sub-2-µm SEC chemistry. The lab equipment has been designed such that it is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons. The total system solution will routinely deliver fast, accurate aggregate determinations that are up to 10 times quicker than the traditional HPLC-based SEC methods. For optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume. This particular listing is a kit that includes the BEH125 SEC Protein Standard Mix (PN: 186006519) at no additional cost. By utilizing the ACQUITY UPLC Protein BEH SEC Column, you can take advantage of some key features such as exceptional improvements in resolution, throughput, and sensitivity for assays. The lab equipment enables you to monitor the presence of protein aggregates through size-exclusion chromatography (SEC), which in turn increases the efficacy as the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics. This combination kit of the ACQUITY UPLC Protein BEH SEC Column column contains the BEH450 SEC Protein Standard Mix, which is a 5 component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil). The Standard Mix has the crucial function of enabling the benchmarking of the ACQUITY UPLC BEH450 SEC, 2.5 µm Columns. This makes it possible for you to conduct regular column benchmarking and system performance checks. It is recommended that these checks be made part of the routine checkups to ensure all equipment is performing adequately. We recommend that you protect your analytical column by using the ACQUITY UPLC Protein BEH SEC Guard Column, 125 Å, 1.7 µm, 4.6 mm X 30 mm, 1/pk . The guard column prevents any harmful contaminants from entering the mobile stream, thus improving the efficiency of the column as well.

FAQ
What Is The Maximum Pressure That The ACQUITY UPLC Protein BEH SEC Column Can Withstand? The ACQUITY UPLC Protein BEH SEC Column listed here is designed to be able to tolerate up to a maximum pressure of 10,000 psi (690 Bar).