Waters, 186002554, SunFire C18 Column, 100Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk

SunFire C18 columns are used for general purpose method development at low pH. The C18 ligand is highly retentive, especially for basic compounds and it is ideally suited for purification and impurity profile assays.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 340
System: HPLC
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 150 mm
Carbon Load: 16 %
UNSPSC: 41115709
Brand: SunFire
Product Type: Columns
Units per Package: 1 pk

Additional Information
SunFire C18 Column, 100Å, 3.5 µm, 4.6 mm X 150 mm, 1/pk Setting the standard for state of the art bonded C18 and C8 silica HPLC columns, SunFire columns are the result of years of in-depth research and product development and represent the best in particle and bonding expertise, delivering industry-leading levels of chromatographic performance. Achieve excellent peak shape, minimal column bleed, and high mass loading with SunFire C18 Columns. Chosen by chromatographers for their resolving power and reliability for analytical and preparative needs, SunFire C18 columns are used for general-purpose method development at low pH. The lifetime of SunFire Columns is extended due to low pH stability when compared with other silica-based HPLC column brands. The columns offer both high efficiency and greater sensitivity as a result of Water’s proprietary and cutting edge silica synthesis, bonding, end-capping, and packing technology procedures. These general-purpose columns are a great choice among the extensive family of Waters’ lab equipment and separation preparation tools. SunFire Silica OBD Prep Column, 100Å, 5 µm, 10 mm X 150 mm, 1/pk and other related products are compatible with mass spectrometry applications, providing sharp peaks, excellent sensitivity, high peak capacity, and ultra-low bleed. By developing new bonding and end-capping technologies, Waters has been able to develop a sorbent with a superior peak shape performance. These columns provide symmetrical peaks that offer improved resolution and quantitation of acidic, neutral, and basic compounds for low to intermediate pH ranges. The use of the C18 ligand allows for high retention particularly for basic compounds, making it ideally suited to purification and impurity profile assays. With the importance of reproducibility in mind, SunFire columns are tested chromatographically with acidic, basic, and neutral analytes, with results held to strict specification ranges. This allows the columns to provide the same chromatographic results over the course of the method and any new drug products created. For analytical methods used in the latest pharmaceutical and biopharmaceutical products, SunFire C18 Columns are able to provide reliable results.

FAQ
Why Is Silica Used In HPLC Columns? Widely accepted as one of the best adsorbents in column chromatography, silica offers a range of benefits, primarily its affinity for absorption. It is also available in a range of forms and sizes and has a lot of research and information on its uses.
Silica is also ideal because it remains neutral and does not interact with any of the substances that pass through a column, allowing it to maintain a stable structure. Silica can be regenerated or reused many times, making purification less costly. It also allows for exact requirements of the particle size to be met.