Waters, 186003561, XBridge Peptide BEH C18 Column, 130Å, 3.5 µm, 1 mm X 100 mm, 1K - 15K, 1/pk

The XBridge BEH130 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 1 mm
Length: 100 mm
Carbon Load: 17 %
UNSPSC: 41115709
Application: Peptide
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Peptide BEH C18 Column, 130Å, 3.5 µm, 1 mm X 100 mm, 1K - 15K, 1/pk Peptide separations are adjusted, quality-controlled, and evaluated using the XBridge BEH130 C18 Peptide Separation Technology (PST) columns. To evaluate samples for proteomics, protein characterization, and peptide synthesis, the small-pore (130) tri-functionally bonded BEH particle offers you the widest usable pH range, improved low pH stability, and ultra-low column bleed. The Waters Peptide Separation Technology (PST), which combines BEH Technology particles, is the basis for the XBridge Peptide BEH C18 Columns. In order to guarantee scientists will obtain stability of peptide separation methods as well as predictable behavior with the range of different samples that it can be used for, such as proteomics, protein characterization, and peptide synthesis, these columns are specially tested with a peptide map. The column will improve the resolution of the complex peptide digests with the addition of BEH Technology. Your choice of peptides will be effectively separated using the XBridge Peptide BEH C18 Column. For the best chromatography, it will also provide you with improved peak shape and retention in formic acid and trifluoroacetic acid. Using The XBridge Peptide BEH C18 Columns, you may seamlessly transition from sub-2 m UPLC Technology to 10 m preparative HPLC separations. The XBridge Peptide BEH C18 Column is delivered in 100% acetonitrile. Prior to switching to a different mobile phase system, it is crucial to confirm mobile phase compatibility. We strongly advise equilibrating the column using at least 10 column volumes of the mobile phase that will be applied. Flush the column with additional five column volumes of a water/organic solvent mixture, use the same or lower solvent concentration as in your preferred buffered mobile phase, and make sure you can prevent precipitating out mobile phase buffers on your column or in your system. Visit our website to learn about other XBridge columns and to shop for Lab Equipment . You might also be interested in the XBridge Peptide BEH C18 OBD Prep Column, 130Å, 10 µm, 10 mm X 250 mm, 1/pk , which offers you the greatest useable pH range, exceptional low pH stability, and ultra-low column bleed to analyze samples for proteomics, protein characterization, and peptide synthesis. It has a small-pore (130) tri-functionally bonded BEH particle.

FAQ
Which Operational Restrictions Apply To The XBridge Peptide BEH C18 Column? Operating the XBridge Peptide BEH C18 Column at temperatures between 20 and 80 degrees Celsius is advised in order to improve selectivity, reduce solvent viscosity, and boost mass transfer rates. When under pressure, the XBridge Peptide BEH C18 Column can withstand 6000 psi (or 415 bar). Depending on the pH and buffer conditions employed, we advise keeping pressure and temperature within the ambient range. Anything outside of those ranges will reduce the column's lifetime.