Waters, 186003581, XBridge Peptide BEH C18 Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1K - 15K, 1/pk

The XBridge BEH130 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 130 Å
QC Tested: Peptide
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 17 %
UNSPSC: 41115709
Application: Peptide
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Peptide BEH C18 Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1K - 15K, 1/pk The XBridge Peptide BEH C18 Columns is based on Waters Peptide Separation Technology (PST), which incorporates BEH Technology particles. These columns are specially tested with a peptide map in order to ensure scientists will get stability of peptide separations methods as well as predictable behavior with the range of different samples that it can be used for-- such as proteomics, protein characterization, and peptide synthesis. With the infused BEH Technology, the column will increase the resolution of the complex peptide digests. The XBridge Peptide BEH C18 Column will give you excellent separation over a wide variety of peptides. It will also give you superior peak shape and retention in formic acid and trifluoroacetic acid for optimal chromatography. You will also be able to get seamless method migration using The XBridge Peptide BEH C18 Columns from sub-2 µm UPLC Technology to 10 µm preparative HPLC separations. XBridge Peptide BEH C18 Column is shipped in 100% acetonitrile. It is important to ensure mobile phase compatibility before changing to a different mobile phase system. We highly recommend to Equilibrate the column with a minimum of 10 column volumes of the mobile phase to be used. To ensure you can avoid precipitating out mobile phase buffers on your column or in your system, flush the column with another five column volumes of a water/organic solvent mixture, using the same or lower solvent content as in your desired buffered mobile phase. Temperatures between 20 - 80 degrees Celsius are recommended for operating the XBridge Peptide BEH C18 Column in order to enhance selectivity, lower solvent viscosity, and increase mass transfer rates. XBridge Peptide BEH C18 Column can tolerate a pressure up to 6000 psi (or 415 bar). We advise maintaining pressure and temperature within the ambient range, as anything over the ranges will have a negative effect on the lifetime of the column depending on the pH and buffer conditions used. You can check out our website to explore other XBridge columns and Shop for Lab Equipment. You might also be interested in the XBridge Peptide BEH C18 OBD Prep Column, 130Å, 10 µm, 10 mm X 250 mm, 1/pk; which has a small-pore (130Å) tri functionally bonded BEH particle that offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

FAQ
What Is The Optimal pH Range For The XBridge Peptide BEH C18? The XBridge Peptide BEH C18 ideally operates within the 1 - 12 pH range. Please keep in mind, using higher pH may impact performance and lifetime of your column.