Waters, 186003748, Atlantis T3 Column, 100Å, 5 µm, 4.6 mm X 250 mm, 1/pk

Atlantis T3 columns are universal, silica-based, reversed-phase C18 columns that provide balanced retention of polar and hydrophobic molecules and should be your first choice when developing separations of polar and non-polar compounds.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 330
System: HPLC
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 14 %
UNSPSC: 41115709
Brand: Atlantis
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about Atlantis T3 Column In what chromatographic conditions is the Atlantis T3 Column particularly effective? The Atlantis T3 Column is particularly effective for the reversed-phase separation of polar and non-polar compounds, offering excellent retention and peak shapes due to its tailored C18 surface chemistry. Can the Atlantis T3 Column be used with aqueous mobile phases? Yes, the Atlantis T3 Column is designed to provide superior performance with aqueous mobile phases, making it suitable for the retention of polar compounds that are often poorly retained on traditional C18 columns. What type of analytes can be separated using the Atlantis T3 Column? The Atlantis T3 Column is versatile and can be used to separate a wide range of analytes, from small polar molecules to larger non-polar compounds, due to its unique trifunctional C18 bonding. How does the Atlantis T3 Column contribute to method development in pharmaceutical analysis? The Atlantis T3 Column’s ability to retain polar compounds makes it a valuable tool in pharmaceutical analysis, especially when developing methods for new chemical entities with unknown retention behavior. What maintenance is required to ensure the Atlantis T3 Column's longevity? Regular maintenance of the Atlantis T3 Column includes proper solvent washing protocols to remove any bound residues after analyses and following the manufacturer's guidelines for storage conditions.

FAQ
What Is An Ion-Pairing Reagent? When samples contain ionic components, they may be too polar to be retained by a reversed-phase mode. In these cases, ion-pairing can be useful. An ion-pairing reagent has an ionic end and a non-polar tail, which is added to the mobile phase and allowed to reach equilibrium with the column. The non-polar end of the reagent is held strongly by the stationary phase (such as C18), leaving the charged functional group sticking out into the mobile phase. This allows ionic species of the opposite charge to be attracted to the immobilized ion-pair reagent, resulting in chromatographic retention.