Waters, 186004870, XBridge BEH Amide Column, 130Å, 3.5 µm, 4.6 mm X 250 mm, 1/pk

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2-11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa.

Specifications
Chemistry: Amide
Separation Mode: Hydrophilic Interaction (HILIC)
Particle Substrate: Hybrid
pH Range Min: 2 pH
pH Range Max: 11 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: No
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 3.5 µm
Endfitting Type: Waters
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: HPLC
Particle Technology: BEH
USP Classification: L68
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 12 %
UNSPSC: 41115709
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge BEH Amide Column, 130Å, 3.5 µm, 4.6 mm X 250 mm, 1/pk Based on Waters’ novel ethylene bridged hybrid (BEH) particle, the XBridge Amide Column utilizes a chemically stable, trifunctionally bonded amide phase to offer unprecedented stability and versatility in HILIC separations lab equipment . This ability is a part of Waters’ innovative approach to overcoming the challenge of retaining and separating extremely polar compounds in HILIC stationary phases. With the same selectivity as ACQUITY UPLC BEH Amide columns, the XBridge BEH Amide Column has allowed scientists to transfer separations seamlessly between HPLC and UPLC technology platforms. The XBridge BEH Amide Column is designed specifically to retain polar analytes and metabolites that are too polar to retain using reversed-phase chromatography and can support the use of a wide range of mobile phase pH, from 2 to 11. This facilitates the exceptional retention of polar analytes spanning a wide range in polarity, structural moiety, and pKa. In addition to enhancing the retention of polar compounds, the column offers an increased mass spectrometry response, direct compatibility with sample preparation eluates like PPT, LLE, and SPE, and orthogonal selectivity when compared to reversed-phase materials. The XBridge BEH Amide Column is particularly suited for the analysis of carbohydrates due to the 3.5 µm particle size, which enables high resolution and high-speed analysis in complex sample matrices while maintaining or improving the chromatographic resolution. Each batch of XBridge material is tested chromatographically with acidic, basic, and neutral analytes, with the results held to narrow specification ranges. All packing materials use ultra-pure reagents. Further validation can be done using HILIC QC Reference Material .

FAQ
How Should Guard Columns Be Used With The XBridge BEH Amide Column? For continued high performance of your XBridge column, use a Waters guard column of matching chemistry and particle size between the injector and the main column. It’s critical to use a high-performance and matching guard column to protect the main column without compromising or changing the analytical resolution.
Guard columns should also be replaced at regular intervals, as determined by sample contaminations. When a steady increase in system backpressure is observed above a set pressure limit, this is often an indicator that guard columns should be swapped out. Sudden appearances of split peaks can also mean this.