Waters, 186009840, XSelect Premier Peptide HSS T3 Column, 100 Å, 2.5 µm, 2.1 x 100 mm

The XSelect Premier Peptide HSS T3 Column, 100 Å, 2.5 µm, 2.1 x 100 mm Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Temperature Limits: 45 C
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Bonding Technology: T3
Silanol Activity: Medium
Particle Shape: Spherical
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 100 Å
QC Tested: Peptide
Format: Premier Column
Surface Area: 230
System: UHPLC, UPLC
Particle Technology: HSS
Technique: LC
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 100 mm
Carbon Load: 11 %
eCord: Yes
UNSPSC: 41115709
Application: Peptide
Brand: XSelect
Product Type: Columns
Units per Package: 1 pk

Additional Information
XSelect Premier Peptide HSS T3 Column, 2.5 µm, 2.1 x 100 mm Designed for separations where silica-based selectivity is desired, the XSelect Premier Peptide HSS T3 Column is a top choice for scientists performing hydrophilic peptide separations or other proteomics-related assays. Thanks to the High-Strength Silica (HSS) particle morphology, this column is equipped to provide superior separation performance resolution when hydrophilic peptide retention is necessary. What’s more, this silica column belongs to the MaxPeak Class of Columns, a designation only given to chromatography consumables that prioritize increased recover for metal-sensitive analytes. MaxPeak technology prevents unwanted analyte-surface interactions from occurring, all leading to a reliable data set that’s easily reproducible. Add the XSelect Premier Peptide HSS T3 Column to your lab’s toolkit today. For excellent reverse-phase retention of hydrophilic peptides to occur, you need a column that provides superior stability across varying backpressures and temperatures. That’s why the XSelect Premier Peptide HSS T3 Column remains a top choice among scientists working in proteomics. The general-purpose nature of the column makes it a flexible tool that can be used with a wide range of analytes. What’s more, this column is 100% water wettable, which means using a 100% aqueous mobile phase is of no great concern to your workflow. This HPLC column can still provide superior separation performance because of that stability. Another huge perk of the XSelect Premier Peptide HSS T3 Column is its ability to help you perform method transfer. It doesn’t matter if you need to use this HPLC column to transfer data to UHPLC or UPLC systems, as it prioritizes reproducibility across different LC systems. This all leads to results you can be incredibly confident in. And with improved peak shape and retentivity for basic compounds, this silica column can be used with different analytes without concerns about overall performance. While the XSelect Premier Peptide HSS T3 Column provides enhanced retention of hydrophilic peptides, we understand that’s not the goal of every scientists. But if you want the separation performance that’s offered by this column, we have good news. Shop XSelect Premier Columns and you’re sure to find the right chromatographic particle and column chemistry to match your assays. For a lab to run as efficiently as possible, scientists need the right equipment and tools at their disposal. Luckily for you, we have it all at Waters. From quality control reference materials and MS systems to lab informatics, you’ll find exactly what you need to be successful in the lab when you shop with us. Shop for lab equipment now to learn more.

FAQ
What’s the Max Pressure Allowed By the XSelect Premier Peptide HSS T3 Column? The XSelect Premier Peptide HSS T3 Column can operate under pressures of up to 18,000 PSI.