Thermo Fisher, 11363D, Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit

Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.
Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remainFoxp3+.
• Treg cells can be used in any downstream applications (e.
g., suppression assays)MethodologyThe isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™Starting samplePBMCCell Type: T Cells (Regulatory)
Isolation Technology: Depletion, then positive isolation
No. of Cells: Processes ∼1 x 109cells total
Output Viability: >95%
Product Line: DETACHaBEAD™, DYNAL™, Dynabeads™, FlowComp™
Purity or Quality Grade: Research Grade
Quantity: 2 x 5 mL
Sample Type: PBMC
Shipping Condition: Room Temperature
Starting Material Cell No.: 1 x 108PBMCs per isolation
Target Species: Human
Product Type: Cell Isolation Kit
Unit Size: Each