Thermo Fisher, 12033015, ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells

ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010cfu/μg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction.
ElectroMAX DH10B cells:
• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applicationsPhage-resistant representative libraries from a single transformationThetonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, theendA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.
GenotypeF-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalK λ-rpsLnupGtonAFind the strain and format that fits your needsDH10B cellsare available in both electrocompetent and chemically competent formats.
ElectroMAX DH10B cellsare suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection.
MegaX DH10B T1RElectrocomp Cellshave the highest transformation efficiency (>3 x 1010cfu/μg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: ElectroMAX™
Product Type: Electrocompetent Cells
Quantity: 5 x 100 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each