Thermo Fisher, 12034013, MAX Efficiency™ DH5α-T1R Competent Cells

MAX Efficiency™ DH5α-T1R Competent Cells MAX Efficiency DH5α T1RPhage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5. In addition to supporting blue/white screening,recA1 andendA1 mutations in DH5α increase insert stability and improve the quality of plasmid DNA prepared from minipreps. These cells are suitable for the construction of gene banks or generation of cDNA libraries using derived vectors.
MAX Efficiency DH5α T1RPhage-Resistant Competent Cells have been prepared by a patented modification of the procedure of Hanahan and yield >1 x 109transformants/μg of monomer pUC19 DNA in a 100 μL transformation reaction.
MAX Efficiency DH5α T1RPhage Resistant Competent Cell features
• Resistance to the lytic bacteriophage T1, one of the most dangerousE. coliphages in high throughput laboratories and genomic centers
• The φ80lacZΔM15 marker that provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and, therefore, can be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal
• Capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 is provided to allow plaque formationGenotypeF-φ80lacZΔM15 Δ(lacZYA-argF)U169recA1endA1hsdR17(rk-, mk+)phoAsupE44 λ-thi-1gyrA96relA1tonAAntibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: MAX Efficiency™
Product Type: Chemically Competent Cells
Quantity: 5 x 200 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each